Department of Biology
Courses Taught at Fredonia
Human Biology – BIOL 110
Research Interests – Microtubule regulation in cancerous and noncancerous cells.
The microtubule network is critical for a number of different functions in the cell, including organelle localization, vesicular trafficking and mitosis. Microtubules themselves must be organized for these tasks to be carried out. During interphase, microtubules are organized around a location known as the Microtubule Organizing Center (MTOC). After microtubules are nucleated at the MTOC, they are retained in position by the microtubule-anchoring complex, retaining them in a radial array. There have been several proteins identified as belonging to the anchoring complex, including the protein dynactin, the required cofactor for the microtubule motor protein dynein. Within dynactin there are two distinct microtubule-binding domains, each with different affinities for microtubules. We are trying to determine which domain is responsible for anchoring and which domain functions in other cellular tasks as well as understand how the cell regulates which domain is active. We are also interested in how microtubules and associated proteins function in spindle pole organization and regulation during cell division in cancer cells. Malfunction at this stage can lead to chromosomal defects, such as lagging chromosomes or multipolar spindles. This in turn can lead to genomic instability, which is a hallmark of tumor progression. We are examining how upregulation or downregulation of specific proteins contribute to these defects so that we can build a full picture of the mechanism by which they contribute to genomic instability.
Quintyne, N. J. and M. M. Ivey. 2012. Discussion, Implementation, Presentation: A standalone course for high ability undergraduate students. Chemical Educator 17: 179-183.
Yeh, T.-Y., N. J. Quintyne, B. R. Scipioni, D. M. Eckley and T. A. Schroer. 2012. Dynactin’s pointed end complex is a cargo-targeting module. Mol. Biol. Cell. 23: 3827-3837. Pubmed
Washington J. T. and N. J. Quintyne. 2012. Dichloroacetate induces differential rates of cell death in both tumor and nontumor cell lines. Tumori. 98: 142-151. Pubmed
Botta G., C. S. Turn, N. J. Quintyne and P. A. Kirchman. 2011. Increased iron supplied through Fet3p results in replicative life span extension of Saccharomyces cerevisiae under conditions requiring respiratory metabolism. Exp Gerontol. 46: 827-832. Pubmed
Culver-Hanlon, T. L., S. A. Lex, A. D. Stephens, N. J. Quintyne, and S. J. King. 2006. Dynactin has two distinct microtubule binding domains. Nat. Cell Bio. 8: 264-270. Pubmed
Quintyne, N. J., J. E. Reing, D. R. Hoffelder, S. M. Gollin and W. S. Saunders. 2005. Spindle Multipolarity is prevented by centrosomal clustering. Science. 307: 127-129. Pubmed
King, S. J., C. L. Brown, K. C. Maier, N. J. Quintyne and T. A. Schroer. 2003. Analysis of the dynein–dynactin interaction in vitro and in vivo. Mol. Biol. Cell. 14: 5089-5097. Pubmed
Quintyne N. J. and T. A. Schroer. 2002. Distinct cell cycle-dependent roles for dynactin and dynein at centrosomes. J. Cell Biol. 159: 245-254. Pubmed
Quintyne, N. J., S. R. Gill, D. M. Eckley, C. L. Crego, D. A. Compton and T. A. Schroer. 1999. Dynactin is required for microtubule anchoring at centrosomes. J. Cell Biol. 147: 321-334. Pubmed
Rachel E. Turn and Nicholas J. Quintyne. Live analysis of vesicular transport in CAD cells: determining the activity of plus- and minus-end directed motors. American Society for Cell Biology, 2012.
Rosa N. Beltran, Joseph D. Williams and Nicholas J. Quintyne. Tracking mitotic defects in oral cancer cells using siRNA-mediated knockdown and live cell analysis. American Society for Cell Biology, 2012.
Kristal M. Hazellief, Christina S. Turn and Nicholas J. Quintyne. Using siRNA to map the contribution of dynactin subunits in anchoring microtubules at the centrosome. American Society for Cell Biology, 2012.
Ana C. Rodrigues and Nicholas J. Quintyne. Using the Oral Cancer Cell line UPCI:SCC078 to purify NuMA protein. American Society for Cell Biology, 2012.
Jordan E. Hoke, Miguel E. Rivera, Alexa M. Billow, Laura Alsina and Nicholas J. Quintyne. Characterization of the kinesin KIF9 in mammalian cell cycle progression. American Society for Cell Biology, 2011.
Ariel G. Le, Rebecca L. Schneider, Lindsay M. McCullough and Nicholas J. Quintyne. Microtubule anchoring is facilitated by the shoulder/sidearm subunits of the dynactin complex. American Society for Cell Biology, 2011.
Isabel S. Griffin, Travis J. Yates and Nicholas J. Quintyne. Antioxidants rescue carcinogen induced mitotic defects in both chromosomally stable and unstable cells. American Society for Cell Biology, 2011.
Lindsay M. McCullough and Nicholas J. Quintyne. Knockdown of dynactin’s p24 subunit leads to defects throughout the cell cycle. American Society for Cell Biology, 2010.
Alexa M. Billow, Laura Alsina and Nicholas J. Quintyne. KIF9 knockdown in mammalian cells leads to multiple mitotic defects. American Society for Cell Biology, 2010.